Unique NS5b hepatitis C virus gene sequence consensus database is essential for standardization of genotype determinations in multicenter epidemiological studies

Syria Laperche (1) , Karine Sandres-Sauné , Paul Dény , Gilles Duverlie , Sophie Alain (2, 3) , Marie-Laure Chaix (4) , Catherine Gaudy , Françoise Lunel , Jean-Michel Pawlotsky , Christopher Payan , Bruno Pozzetto (5) , Catherine Tamalet , Vincent Thibault , Sophie Vallet , Françoise Bouchardeau , Jacques Izopet (6) , Jean-Jacques Lefrère

1 Centre National de Référence Virus des hépatites B, C et Delta
2 UNILIM - Université de Limoges
3 EA3175 - Biologie moléculaire et cellulaire des microorganismes
4 Laboratoire de Virologie [CHU Necker]
5 GIMAP - Groupe Immunité des Muqueuses et Agents Pathogènes
6 Laboratoire Virologie [CHU Toulouse]

A multicenter study of NS5b hepatitis C virus (HCV) genotype determination involving 12 laboratories demonstrates that any laboratory with expertise in sequencing techniques would be able to provide a reliable HCV genotype for clinical and epidemiological purposes as long as they are provided a consensus reference sequence database.

Domaines: Sciences du Vivant [q-bio]; Microbiologie et Parasitologie; Bactériologie; Sciences du Vivant [q-bio]; Microbiologie et Parasitologie; Virologie; Sciences du Vivant [q-bio]; Médecine humaine et pathologie; Maladies infectieuses

Syria Laperche, Karine Sandres-Sauné, Paul Dény, Gilles Duverlie, Sophie Alain, et al.. Unique NS5b hepatitis C virus gene sequence consensus database is essential for standardization of genotype determinations in multicenter epidemiological studies. Journal of Clinical Microbiology, 2006, 44 (2), pp.614-616. ⟨10.1128/JCM.44.2.614-616.2006⟩. ⟨hal-00405909⟩