Insertion sequence 1515 in the ply gene of a type 1 clinical isolate of Streptococcus pneumoniae abolishes pneumolysin expression

Fabien Garnier (1, 2) , Rajendra Prasad Janapatla , Emmanuelle Charpentier , Geoffrey Masson , Carole Grélaud , Jean François Stach , François Denis (2, 3) , Marie-Cécile Ploy (2, 3)

1 Service de Bactériologie, Virologie, Hygiène [CHU Limoges]
2 EA3175 - Biologie moléculaire et cellulaire des microorganismes
3 UNILIM - Université de Limoges

A serotype 1 Streptococcus pneumoniae strain isolated by blood culture from a woman with pneumonia was found to harbor insertion sequence (IS) 1515 in the pneumolysin gene, abolishing pneumolysin expression. To our knowledge, this is the first report of an IS in the pneumolysin gene of S. pneumoniae.

Domaines: Sciences du Vivant [q-bio]; Microbiologie et Parasitologie; Bactériologie; Sciences du Vivant [q-bio]; Microbiologie et Parasitologie; Virologie; Sciences du Vivant [q-bio]; Médecine humaine et pathologie; Maladies infectieuses

Fabien Garnier, Rajendra Prasad Janapatla, Emmanuelle Charpentier, Geoffrey Masson, Carole Grélaud, et al.. Insertion sequence 1515 in the ply gene of a type 1 clinical isolate of Streptococcus pneumoniae abolishes pneumolysin expression. Journal of Clinical Microbiology, 2007, 45 (7), pp.2296-2297. ⟨10.1128/JCM.02168-06⟩. ⟨hal-00406730⟩