Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Double immunohistochemistry with horseradish peroxidase and alkaline phosphatase detection systems.

Abstract : We describe here a protocol optimized for formalin-fixed paraffin-embedded tissue sections that enables the detection of two antigens. This technique allows immunohistochemistry to be performed with detection systems allowing observation by light microscopy. This chapter discusses the choice of appropriate protocols as well as the choice of visualization systems.In doing so, we provide examples of representative results obtained with this protocol and describe necessary controls; additionally, we discuss common problems associated with this methodology, and detail troubleshooting recommendations. Although this method has been optimized for liver sections, it may be applicable for performing double immunostaining in a variety of tissue samples.
Liste complète des métadonnées

https://hal-unilim.archives-ouvertes.fr/hal-00594289
Contributeur : Franck Sturtz <>
Soumis le : jeudi 19 mai 2011 - 14:25:27
Dernière modification le : lundi 2 juillet 2018 - 16:26:04

Identifiants

Collections

Citation

Vincent Sarrazy, Alexis Desmoulière. Double immunohistochemistry with horseradish peroxidase and alkaline phosphatase detection systems.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2010, 611, pp.59-71. ⟨10.1007/978-1-60327-345-9_5⟩. ⟨hal-00594289⟩

Partager

Métriques

Consultations de la notice

171