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Development of a downscale sedimentation field flow fractionation device for biological event monitoring.

Abstract : Classically described as a macroscale size-density based method, Sedimentation field flow fractionation (SdFFF) has been successfully used for cell sorting. The goal of this study was to develop a new SdFFF device for downscale applications, in particular for oncology research to rapidly monitor chemical biological event induction in a cell line. The development of a downscale SdFFF device required reduction of the separation channel volume. Taking advantage of a newly laboratory designed apparatus, channel volume was successfully decreased by reducing both length and breadth. To validate the apparatus and method, we used the well-known model of diosgenin dose-dependent induction of apoptosis or megakaryocytic differentiation in HEL cells. After a minute scale acquisition of a reference profile, the downscale device was able to perform fast, early, significant and reproducible monitoring of apoptosis and differentiation, two important biological mechanisms in the field of cancer research.
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https://hal-unilim.archives-ouvertes.fr/hal-00595226
Contributeur : Françoise Merigaud <>
Soumis le : mardi 24 mai 2011 - 11:05:03
Dernière modification le : mardi 20 mars 2018 - 16:36:29

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Gaëlle Bégaud-Grimaud, Bertrand Liagre, Serge Battu, Jean-Louis Beneytout, Philippe Cardot, et al.. Development of a downscale sedimentation field flow fractionation device for biological event monitoring.. Journal of Chromatography A, Elsevier, 2009, 1216 (52), pp.9125-33. ⟨10.1016/j.chroma.2009.08.042⟩. ⟨hal-00595226⟩

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