Analysis of relationship between cell cycle stage and apoptosis induction in K562 cells by sedimentation field-flow fractionation. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences Année : 2009

Analysis of relationship between cell cycle stage and apoptosis induction in K562 cells by sedimentation field-flow fractionation.

(1) , (2) , (1, 3) , (1, 4, 5) , (2) , (1, 5) , (1, 5)
1
2
3
4
5

Résumé

Recently, sedimentation field-flow fractionation (SdFFF) was used to study the specific kinetics of diosgenin-induced apoptosis in K562 cells. Here, we propose a new SdFFF cell separation application in the field of cancer research concerning the correlation between induction of a biological event (i.e. apoptosis) and cell status (i.e. cell cycle position). SdFFF isolated subpopulations depending on the cell cycle position allowing the study of apoptosis kinetics and extent. Results showed that cells in G0/G1 phases (F3 cells) underwent significant and earlier apoptosis than cells in the active part of the cell cycle (S/G2/M phases). Results shed light on the correlation between differences in apoptosis kinetics and cell cycle stage when exposure to the inducer began. SdFFF monitoring and size measurement also led to the description of different subpopulations demonstrating complex variations in density between fractions associated with differences in biological processes.
Fichier non déposé

Dates et versions

hal-00595229 , version 1 (24-05-2011)

Identifiants

Citer

Julian Bertrand, Bertrand Liagre, Gaëlle Bégaud-Grimaud, Marie-Odile Jauberteau-Marchan, Jean-Louis Beneytout, et al.. Analysis of relationship between cell cycle stage and apoptosis induction in K562 cells by sedimentation field-flow fractionation.. Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, 2009, 877 (11-12), pp.1155-1161. ⟨10.1016/j.jchromb.2009.02.064⟩. ⟨hal-00595229⟩
75 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook Twitter LinkedIn More