Automatic cytometric device using multiple wavelength excitations

Nelly Rongeat (1) , S. Ledroit , L. Chauvet , D. Cremien , Alexandra Urankar (2) , Vincent Couderc (1) , P. Nerin

1 PHOTONIQUE
2 FT R&D - France Télécom Recherche & Développement

Precise identification of eosinophils, basophils, and specific subpopulations of blood cells (B lymphocytes) in an unconventional automatic hematology analyzer is demonstrated. Our specific apparatus mixes two excitation radiations by means of an acousto-optics tunable filter to properly control fluorescence emission of phycoerythrin cyanin 5 (PC5) conjugated to antibodies (anti-CD20 or anti-CRTH2) and Thiazole Orange. This way our analyzer combining techniques of hematology analysis and flow cytometry based on multiple fluorescence detection, drastically improves the signal to noise ratio and decreases the spectral overlaps impact coming from multiple fluorescence emissions.

Domaines: Sciences du Vivant [q-bio]; Biochimie, Biologie Moléculaire; Sciences du Vivant [q-bio]; Ingénierie biomédicale; Médecine nucléaire

Nelly Rongeat, S. Ledroit, L. Chauvet, D. Cremien, Alexandra Urankar, et al.. Automatic cytometric device using multiple wavelength excitations. Journal of Biomedical Optics, 2011, 16 (5), pp.057006. ⟨10.1117/1.3582162⟩. ⟨hal-00643743⟩