Accéder directement au contenu Accéder directement à la navigation
Communication dans un congrès

Automatic cytometric device using multiple wavelength excitations

Abstract : Precise identification of eosinophils, basophils, and specific subpopulations of blood cells (B lymphocytes) in an unconventional automatic hematology analyzer is demonstrated. Our specific apparatus mixes two excitation radiations by means of an acousto-optics tunable filter to properly control fluorescence emission of phycoerythrin cyanin 5 (PC5) conjugated to antibodies (anti-CD20 or anti-CRTH2) and Thiazole Orange. This way our analyzer combining techniques of hematology analysis and flow cytometry based on multiple fluorescence detection, drastically improves the signal to noise ratio and decreases the spectral overlaps impact coming from multiple fluorescence emissions.
Type de document :
Communication dans un congrès
Liste complète des métadonnées

https://hal-unilim.archives-ouvertes.fr/hal-00646112
Contributeur : Véronique Maury <>
Soumis le : mardi 29 novembre 2011 - 11:29:31
Dernière modification le : jeudi 11 janvier 2018 - 06:26:10

Identifiants

  • HAL Id : hal-00646112, version 1

Collections

Citation

Nelly Rongeat, Sylvain Ledroit, Didier Cremien, Alexandra Urankar, Laurence Chauvet, et al.. Automatic cytometric device using multiple wavelength excitations. European Conferences on Biomedical Optics, présentation orale [8087-62], May 2011, Munich, Germany. ⟨hal-00646112⟩

Partager

Métriques

Consultations de la notice

203