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Article Dans Une Revue Analytical Methods Année : 2010

Progress curve analysis of enzyme reactions by flow microcalorimetry: Use of a pseudo-first order approximation

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Résumé

We describe a method for determining the pseudo-first order rate constant of a chemical reaction by flow microcalorimetry operating in the flow-through mode. The impulse response of the instrument was described by a Gamma distribution and the equation of the thermogram was computed analytically. The resulting equation was fitted to the data by simulated annealing. The method was applied to an enzyme reaction following Michaelis-Menten kinetics by means of a new empirical equation relating the apparent rate constant to the kinetic parameters Vmax and Km. The method was exemplified by a kinetic study of horse serum butyrylcholinesterase.

Dates et versions

hal-00651681 , version 1 (14-12-2011)

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Jean Debord, Michel Harel, Jean-Claude Bollinger, Thierry Dantoine. Progress curve analysis of enzyme reactions by flow microcalorimetry: Use of a pseudo-first order approximation. Analytical Methods, 2010, 2 (12), pp.1958-1961. ⟨10.1039/C0AY00223B⟩. ⟨hal-00651681⟩
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