Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry.

Souad Hadjoudja; Chantal Vignoles; Véronique Deluchat; Jean-François Lenain; Anne-Hélène Le Jeune; Michel Baudu

Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry.

Souad Hadjoudja Chantal Vignoles Véronique Deluchat ¹ Jean-François Lenain ¹ Anne-Hélène Le Jeune ^{2, 3} Michel Baudu ¹

1 GRESE - Groupement de Recherche Eau, Sol, Environnement

2 LMGE - Laboratoire Microorganismes : Génome et Environnement

3 LSEE - Laboratoire des Sciences de l'Eau et de l'Environnement

Abstract : Copper sulphate is a common algaecide applied to ponds in order to keep phytoplanktonic blooms under control, especially those prone to cyanobacterial development. The copper toxicity was evaluated for two phytoplanktonic species with the same size and shape: one cyanobacteria Microcystis aeruginosa and one chlorophyceae Chlorella vulgaris. Under controlled conditions in order to keep free copper concentration constant, three bioassays were carried out: growth inhibition tests and two sets of flow cytometric analysis (autofluorescence and esterase activity inhibition). The results showed: (1) that regardless of the cell type, as copper concentrations increased, cell division rate and autofluorescence decreased, (2) a difference in sensitivity according to the length of exposure to copper: 24 or 48 h, and (3) that M. aeruginosa showed a greater sensitivity than C. vulgaris in the 24h copper exposure assay according to esterase activity data.

Type de document :

Article dans une revue

Domaine :

Sciences de l'environnement

Sciences du Vivant [q-bio] / Biochimie, Biologie Moléculaire / Biochimie [q-bio.BM]

Liste complète des métadonnées

https://hal-unilim.archives-ouvertes.fr/hal-00653617
Contributeur : Françoise Merigaud <>
Soumis le : lundi 19 décembre 2011 - 18:21:06
Dernière modification le : lundi 24 septembre 2018 - 09:42:14

Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry.

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Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry.

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