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Microchamber Set-up Characterization for Nanosecond Pulsed Electric Field Exposure

Abstract : Intracellular structures of biological cells can be disturbed by exposure to nanosecond pulsed electric field (nsPEF). A microchamber-based delivery system mounted on a microscope setup for real-time exposure to nsPEF is studied in this paper. A numerical and experimental characterization of the delivery system is performed both in frequency and time domains. The microchamber delivery system presents a high impedance compared to classical 50 Ω loads. Its frequency behavior and limits are investigated using an in-house finite-difference time-domain (FDTD) simulator and through experimental measurements. High-voltage measurements for two nsPEF generators are carried out. The applied pulse voltage measured across the microchamber electrodes is ~1 kV, corresponding to ~10 MV/m electric fields in the microchamber. Depending on the nsPEF generator used, the measured pulse durations are equal to 3.0 and 4.2 ns, respectively. The voltage distribution provided by FDTD simulations indicates a good level of homogeneity across the microchamber electrodes. Experimental results include permeabilization of biological cells exposed to 3.0-ns, 10-MV/m PEFs.
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Contributeur : Véronique Maury <>
Soumis le : lundi 27 février 2012 - 16:08:44
Dernière modification le : jeudi 11 janvier 2018 - 06:26:09

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Delia Arnaud-Cormos, Philippe Lévêque, Y.H. Wu, J.M. Sanders, M.A. Gundersen, et al.. Microchamber Set-up Characterization for Nanosecond Pulsed Electric Field Exposure. IEEE Transactions on Biomedical Engineering, Institute of Electrical and Electronics Engineers, 2011, 58 (6), pp.1656-1662. ⟨10.1109/TBME.2011.2108298⟩. ⟨hal-00674576⟩

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