Genotyping of Toxoplasma gondii isolates with 15 microsatellite markers in a single multiplex PCR assay.

Daniel Ajzenberg (1, 2, 3) , Frédéric Collinet (1) , Aurélien Mercier (3) , Philippe Vignoles (3, 1) , Marie-Laure Dardé (1, 2, 3)

1 UNILIM - Université de Limoges
2 CNR Toxoplasmose-Toxoplasma BRC - Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Resource Center (BRC)
3 NETEC - Neuroépidémiologie Tropicale et Comparée

We developed an easy-to-use method for genotyping Toxoplasma gondii isolates in a single multiplex PCR assay with 15 microsatellite markers. This method was validated by testing 26 reference isolates that had been characterized with other sets of markers.

Domaines: Santé publique et épidémiologie

Daniel Ajzenberg, Frédéric Collinet, Aurélien Mercier, Philippe Vignoles, Marie-Laure Dardé. Genotyping of Toxoplasma gondii isolates with 15 microsatellite markers in a single multiplex PCR assay.. Journal of Clinical Microbiology, 2010, 48 (12), pp.4641-5. ⟨10.1128/JCM.01152-10⟩. ⟨hal-00677196⟩