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Identification of surface proteins involved in the adhesion of a probiotic Bacillus cereus strain to mucin and fibronectin
Abstract : Several Bacillus strains isolated from commercial probiotic preparations were identified at the species level, and their adhesion capabilities to three different model intestinal surfaces (mucin, Matrigel and Caco-2 cells) were assessed. In general, adhesion of spores was higher than that of vegetative cells to the three matrices, and overall strain Bacillus cereusCH displayed the best adhesion. Different biochemical treatments revealed that surface proteins of B. cereusCH were involved in the adhesion properties of the strain. Surface-associated proteins from vegetative cells and spores of B. cereusCH were extracted and identified, and some proteins such as S-layer components, flagellin and cell-bound proteases were found to bind to mucin or fibronectin. These facts suggest that those proteins might play important roles in the interaction of this probiotic Bacillus strain within the human gastrointestinal tract.
https://hal-unilim.archives-ouvertes.fr/hal-00697291
Contributeur : Claire Douady Connectez-vous pour contacter le contributeur
Soumis le : mardi 15 mai 2012 - 10:00:04
Dernière modification le : lundi 20 décembre 2021 - 16:44:01
Contributeur : Claire Douady Connectez-vous pour contacter le contributeur
Soumis le : mardi 15 mai 2012 - 10:00:04
Dernière modification le : lundi 20 décembre 2021 - 16:44:01
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