Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates.

Philippe Lanotte (1, 2) , Chloé Plouzeau (3) , Christophe Burucoa (3, 4) , Carole Grélaud (5) , Sophie Guillot (6, 7) , Nicole Guiso (6, 7) , Fabien Garnier (5, 8)

1 Service de Bactériologie-Virologie-Hygiène [Tours]
2 UT - Université de Tours
3 Laboratoire de Bactériologie, CHU de Poitiers
4 UP - Université de Poitiers = University of Poitiers
5 Service de Bactériologie, Virologie, Hygiène [CHU Limoges]
6 CNR - Centre National de Référence de la Coqueluche et autres bordetelloses
7 Prévention et Thérapie Moléculaires des Maladies humaines
8 EA3175 - Biologie moléculaire et cellulaire des microorganismes

We evaluated the performances of 4 commercial real-time PCR kits for Bordetella pertussis IS481 sequence detection in nasopharyngeal aspirates by comparison with an in-house real-time PCR assay. Among them, the Simplexa Bordetella pertussis/parapertussis assay (Focus Diagnostics), the SmartCycler Bordetella pertussis/parapertussis assay (Cepheid), and Bordetella R-gene (Argene) present sensitivities over 90%. One kit proved unsuitable for routine clinical use.

Mots clés: HOLMESII

Mots clés: PERTUSSIS; PARAPERTUSSIS; DIAGNOSIS

Domaines: Microbiologie et Parasitologie

Philippe Lanotte, Chloé Plouzeau, Christophe Burucoa, Carole Grélaud, Sophie Guillot, et al.. Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates.. Journal of Clinical Microbiology, 2011, 49 (11), pp.3943-6. ⟨10.1128/JCM.00335-11⟩. ⟨hal-00739029⟩