Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Clinical Microbiology Année : 2011

Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates.

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Résumé

We evaluated the performances of 4 commercial real-time PCR kits for Bordetella pertussis IS481 sequence detection in nasopharyngeal aspirates by comparison with an in-house real-time PCR assay. Among them, the Simplexa Bordetella pertussis/parapertussis assay (Focus Diagnostics), the SmartCycler Bordetella pertussis/parapertussis assay (Cepheid), and Bordetella R-gene (Argene) present sensitivities over 90%. One kit proved unsuitable for routine clinical use.

Dates et versions

hal-00739029 , version 1 (05-10-2012)

Identifiants

Citer

Philippe Lanotte, Chloé Plouzeau, Christophe Burucoa, Carole Grélaud, Sophie Guillot, et al.. Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates.. Journal of Clinical Microbiology, 2011, 49 (11), pp.3943-6. ⟨10.1128/JCM.00335-11⟩. ⟨hal-00739029⟩
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