Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

[Molecular strain typing contribution to epidemiology of tuberculosis in Limousin (1998 to 2006)] : Intérêt du typage moléculaire pour l'épidémiologie de la tuberculose en Limousin (1998-2006)

Abstract : OBJECTIVES: We conducted a molecular epidemiology of Mycobacterium tuberculosis in Limousin, a French area with a low incidence of tuberculosis (4.8/100,000 inhabitants in 2005) to define the molecular diversity and the pattern of transmission. DESIGN: Two hundred and fifty-nine strains were isolated (each strain corresponds to one patient) from 1998 to 2006. Both spoligotyping and MIRU15 were chosen for our study because of their discriminatory power. RESULTS: Only 165 medical records were available: 99M/66F, mean age 56.4 years (14-94), 32.7% foreign-born patients, 16.9% homeless or living in shelters, 21.8% of immunocompromised patients (three HIV positive), 14.5% of alcohol addicts. Pulmonary manifestations were predominant (81.8%) with 45.1% of positive smears. Two strains among the 259 presented a multidrug resistance. Spoligotyping identified 136/259 spoligotypes (110 unique, 26 clusters composed of two to 36 isolates); within these 26 clusters, ST53 (n=36) and ST50 (n=19) were the most frequent. Three major families were observed as follow: T1 (30%), Haarlem (30%) and LAM (20%). MIRU15 identified 28/36 isolates in the ST53 group and 14/19 in the ST50 group. Eleven clusters (32 strains) with identical ST-MIRU15 were obtained with a proved case of recent transmission. Alcohol dependence, immunosuppression and pulmonary infections seem to be involved in transmission factors. CONCLUSION: M. tuberculosis strains isolated in Limousin are characterized by their high genetic diversity. The rate of recent transmission (8.1%) is low and therefore a reactivation process is predominant in this area.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal-unilim.archives-ouvertes.fr/hal-00742153
Contributeur : Claire Douady <>
Soumis le : mardi 16 octobre 2012 - 09:48:31
Dernière modification le : mercredi 14 octobre 2020 - 03:47:48

Identifiants

Collections

Citation

Holy Bezanahary, M.-C. Baclet, Christophe Sola, Virgile Gazaille, Pascal Turlure, et al.. [Molecular strain typing contribution to epidemiology of tuberculosis in Limousin (1998 to 2006)] : Intérêt du typage moléculaire pour l'épidémiologie de la tuberculose en Limousin (1998-2006). Médecine et Maladies Infectieuses, Elsevier Masson, 2008, 38 (6), pp.309-317. ⟨10.1016/j.medmal.2008.02.008⟩. ⟨hal-00742153⟩

Partager

Métriques

Consultations de la notice

233