Improved sedimentation field-flow fractionation separation channel for concentrated cellular elution.

Carole Mélin; Aurélie Lacroix; Fabrice Lalloué; Arnaud Pothier; L. Y. Zhang; Aurélie Perraud; Claire Dalmay; Christophe Lautrette; Marie-Odile Jauberteau-Marchan; Philippe Cardot; Muriel Mathonnet; Serge Battu

doi:10.1016/j.chroma.2013.05.067

Article dans une revue

Improved sedimentation field-flow fractionation separation channel for concentrated cellular elution.

Carole Mélin Aurélie Lacroix Fabrice Lalloué ^{1, 2} Arnaud Pothier ³ L. Y. Zhang Aurélie Perraud Claire Dalmay ³ Christophe Lautrette Marie-Odile Jauberteau-Marchan ^{1, 4} Philippe Cardot ^{1, 2} Muriel Mathonnet ^{1, 5} Serge Battu ^{1, 2}

1 HCP - Homéostasie Cellulaire et Pathologies

2 UNILIM - Université de Limoges

3 XLIM-MINACOM - MINACOM

XLIM - XLIM

4 Service d'Immunologie et immunogénétique [CHU Limoges]

5 Service de Chirurgie digestive, endocrinienne et générale [CHU Limoges]

Abstract : SdFFF is now commonly used for cell sorting. Nevertheless, as with many other separation methods, SdFFF Hyperlayer elution leads (1) to sample dilution resulting in cell loss which could restrict further use; and (2) to a high output flow rate impacting detector sensitivity and selectivity. In order to limit these problems, we proposed modifications of the SdFFF separation channel consisting both in downscaling and the insertion of an outlet stream splitter. This last system corresponded to a strip which divides the flow rate output into two parts, one containing concentrated cells in a reduced volume and flow rate, the other containing the excess mobile phase useless for further cell manipulation, detection and characterization. For the first time we have shown that splitter implementation and downscaling respected channel flowing and resulted in Hyperlayer elution of around 95% of cells in less than 50% of input flow rate. Improved cell sorting was demonstrated by enrichment (∼10 times) of cancer stem cells from WiDr cells with two times less quantity of injected cells.

Type de document :

Article dans une revue

Domaine :

Sciences du Vivant [q-bio] / Biologie cellulaire

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https://hal-unilim.archives-ouvertes.fr/hal-00872211
Contributeur : Philippe Sindou <>
Soumis le : vendredi 11 octobre 2013 - 14:25:52
Dernière modification le : vendredi 20 décembre 2019 - 15:42:09

Improved sedimentation field-flow fractionation separation channel for concentrated cellular elution.

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Improved sedimentation field-flow fractionation separation channel for concentrated cellular elution.

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