Validation of the analytical procedure for the determination of the neurotoxin β-N-methylamino-L-alanine in complex environmental samples.

Audrey Combes; Saïda El Abdellaoui; Cédric Sarazin; Jérome Vial; Annick Mejean; Olivier Ploux; Valérie Pichon; Bmaals Group

Validation of the analytical procedure for the determination of the neurotoxin β-N-methylamino-L-alanine in complex environmental samples.

Audrey Combes ¹ Saïda El Abdellaoui ¹ Cédric Sarazin ¹ Jérome Vial ¹ Annick Mejean ² Olivier Ploux ² Valérie Pichon ^{1, *} Bmaals Group ³

* Auteur correspondant

1 LECA - Laboratoire Environnement et chimie analytique

2 LCMCP (site Paris VI) - Laboratoire de Chimie de la Matière Condensée de Paris (site Paris VI)

3 NET - Neuroépidémiologie Tropicale

Abstract : The neurotoxic l-2-amino-3-methylaminopropionic acid (BMAA) was hypothesized to be involved in sporadic cases of amyotrophic lateral sclerosis (ALS). Studies highlighting a possible implication of environmental factors in the incidence of sporadic ALS have become more numerous over recent years. Over the past years, the most widely used method for quantifying BMAA was based on the derivatization of this polar and basic molecule with a fluorescent compound (6-aminoquinolonyl-N-hydroxysuccinimidyl, 6-AQC). This derivatization allows the retention of the conjugate by reversed-phase liquid chromatography and its detection by fluorescence. Nevertheless, recent findings have shown that this method applied to complex samples may cause false positive responses. We therefore developed an analytical procedure for the determination of underivatized BMAA at trace level in complex environmental matrices (river water, cyanobacteria and biofilm) using solid-phase extraction (SPE) based on mixed mode sorbent to concentrate and clean up real samples. Analyzes were performed by hydrophilic interaction chromatography (HILIC) coupled to electrospray ionization and tandem mass spectrometry used in multiple reaction monitoring scan mode. Analytical procedures were validated for the different natural samples using the total error approach. BMAA can be quantified by these reliable and highly selective analytical methods in a range of only a few ng mL(-1) in river water and a few ng mg(-1) dry weight in cyanobacteria and biofilm matrices.

Type de document :

Article dans une revue

Domaine :

Sciences du Vivant [q-bio] / Santé publique et épidémiologie

Liste complète des métadonnées

https://hal-unilim.archives-ouvertes.fr/hal-00927701
Contributeur : Elisabeth Grelier <>
Soumis le : lundi 13 janvier 2014 - 14:54:01
Dernière modification le : jeudi 4 juillet 2019 - 11:00:03

Validation of the analytical procedure for the determination of the neurotoxin β-N-methylamino-L-alanine in complex environmental samples.

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Validation of the analytical procedure for the determination of the neurotoxin β-N-methylamino-L-alanine in complex environmental samples.

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