The production of mammalian trematode infective stages by the snail Galba truncatula. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Helminthology Année : 2012

The production of mammalian trematode infective stages by the snail Galba truncatula.

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Daniel Rondelaud
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Roselyne Mouzet
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Philippe Vignoles
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Gilles Dreyfuss
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J. Cabaret
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Résumé

Several experiments on the breeding of trematode-infected Galba truncatula for obtaining and packaging Fasciola hepatica and Paramphistomum daubneyi metacercariae were carried out to determine the more convenient methods to use for commercial production of these infective stages. Compared to the breeding of infected snails in aquaterraria, the use of 14-cm Petri dishes allowed a greater prevalence of snail infection and a higher number of metacercariae. The production of these larvae was still 2.3-3.4 times greater if infected snails were dissected during the patent period. The aspiration of these metacercariae at the extremity of a Pasteur pipette significantly shortens the time necessary for their transfer from Petri dishes to Eppendorf tubes. Using 14-cm Petri dishes, snail dissection and metacercarial aspiration for their transfer strongly reduce the cost price for metacercarial production of the trematodes Fasciola hepatica and Paramphistomum daubneyi.
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Dates et versions

hal-00934687 , version 1 (22-01-2014)

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Daniel Rondelaud, Roselyne Mouzet, Philippe Vignoles, Gilles Dreyfuss, J. Cabaret. The production of mammalian trematode infective stages by the snail Galba truncatula.. Journal of Helminthology, 2012, 88 (1), pp.1-7. ⟨10.1017/S0022149X1200082X⟩. ⟨hal-00934687⟩

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