Neurocysticercosis: HP10 antigen detection is useful for the follow-up of the severe patients.

Agnès Fleury; Esperanza Garcia; Marisela Hernández; Roger Carrillo; Tzipe Govezensky; Gladis Fragoso; Edda Sciutto; Leslie J S Harrison; R Michael Evans Parkhouse

doi:10.1371/journal.pntd.0002096

Neurocysticercosis: HP10 antigen detection is useful for the follow-up of the severe patients.

Agnès Fleury ^{1, *} Esperanza Garcia Marisela Hernández Roger Carrillo Tzipe Govezensky Gladis Fragoso Edda Sciutto Leslie J S Harrison R Michael Evans Parkhouse ²

* Auteur correspondant

1 NET - Neuroépidémiologie Tropicale

2 IGC - Instituto Gulbenkian de Ciência [Oeiras]

Abstract : BACKGROUND: The most severe clinical form of neurocysticercosis (NC) occurs when cysticerci are located in the subarachnoid space at the base of the brain (SaB). The diagnosis, monitoring and treatment of NC-SaB, constitutes a severe clinical challenge. Herein we evaluate the potential of the HP10 antigen detection enzyme-linked immunosorbent assay (HP10 Ag-ELISA) in the long term follow-up of NC-SaB cases. Assay performance was compared with that of Magnetic Resonance Imaging (MRI). In addition, the robustness of the HP10 Ag-ELISA was evaluated independently at two different institutions. METHODOLOGY/PRINCIPAL FINDINGS: A double-blind prospective cohort trial was conducted involving 38 NC-SaB cases and a total of 108 paired serum and cerebrospinal fluid (CSF) samples taken at intervals of 4 to 8 months for up to 43 months. At each medical visit, results of sera and CSF HP10 Ag-ELISA and MRI obtained at last visit were compared and their accuracy was evaluated retrospectively, considering radiological evolution between appointments. In the long-term follow-up study, HP10 Ag-ELISA had a better agreement than MRI with retrospective radiological evaluation. High reproducibility of HP10 Ag-ELISA between laboratories was also demonstrated. CONCLUSIONS: Results reported in this study establish for the first time the usefulness of the comparatively low cost HP10 Ag-ELISA for long term follow-up of NC-SaB patients.

Type de document :

Article dans une revue

Domaine :

Sciences du Vivant [q-bio] / Santé publique et épidémiologie

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https://hal-unilim.archives-ouvertes.fr/hal-00935331
Contributeur : Elisabeth Grelier <>
Soumis le : jeudi 23 janvier 2014 - 13:29:44
Dernière modification le : lundi 28 octobre 2019 - 12:26:08

Neurocysticercosis: HP10 antigen detection is useful for the follow-up of the severe patients.

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