Detection of Toxoplasma gondii DNA in horse meat from supermarkets in France and performance evaluation of two serological tests.

Abdelkrim Aroussi; Philippe Vignoles; François Dalmay; Laurence Wimel; Marie-Laure Dardé; Aurélien Mercier; Daniel Ajzenberg

Detection of Toxoplasma gondii DNA in horse meat from supermarkets in France and performance evaluation of two serological tests.

Abdelkrim Aroussi ¹ Philippe Vignoles ² François Dalmay ^{1, 3} Laurence Wimel ⁴ Marie-Laure Dardé ^{1, 2, 5} Aurélien Mercier ¹ Daniel Ajzenberg ^{1, 2, 5, *}

* Auteur correspondant

1 NET - Neuroépidémiologie Tropicale

2 UNILIM - Université de Limoges

3 Laboratoire de Biostatistique et d'Informatique Médicale

4 Institut Français du Cheval et de l'Equitation

5 CNR Toxoplasmose-Toxoplasma BRC - Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Resource Center (BRC)

Abstract : In France, some cases of severe toxoplasmosis have been linked to the consumption of horse meat that had been imported from the American continent where atypical strains of Toxoplasma gondii are more common than in Europe. Many seroprevalence studies are presented in the literature but risk assessment of T. gondii infection after horse meat consumption is not possible in the absence of validated serological tests and the unknown correlation between detection of antibodies against T. gondii and presence of tissue cysts. We performed magnetic-capture polymerase chain reaction (MC-PCR) to detect T. gondii DNA in 231 horse meat samples purchased in supermarkets in France and evaluated the performance and level of agreement of the modified agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA) in the meat juices. The serological tests lacked sensitivity, specificity, and agreement between them, and there was no correlation with the presence of T. gondii DNA in horse meat, raising concerns about the reliability of T. gondii seroprevalence data in horses from the literature. T. gondii DNA was detected in 43% of horse meat samples but the absence of strain isolation in mice following inoculation of more than 100 horse meat samples suggests a low distribution of cysts in skeletal muscles and a low risk of T. gondii infection associated with horse meat consumption. However, to avoid any risk of toxoplasmosis, thorough cooking of horse meat is recommended.

Type de document :

Article dans une revue

Domaine :

Sciences du Vivant [q-bio] / Santé publique et épidémiologie

Liste complète des métadonnées

https://hal-unilim.archives-ouvertes.fr/hal-01222112
Contributeur : Elisabeth Grelier <>
Soumis le : jeudi 29 octobre 2015 - 11:23:25
Dernière modification le : vendredi 22 mars 2019 - 12:26:16

Detection of Toxoplasma gondii DNA in horse meat from supermarkets in France and performance evaluation of two serological tests.

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Detection of Toxoplasma gondii DNA in horse meat from supermarkets in France and performance evaluation of two serological tests.

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