Synthesis of a molecularly imprinted sorbent for selective solid-phase extraction of β-N-methylamino-l-alanine.

Pavel Svoboda; Audrey Combes; Julia Petit; Lucie Nováková; Valérie Pichon; Bmaals Group

Synthesis of a molecularly imprinted sorbent for selective solid-phase extraction of β-N-methylamino-l-alanine.

Pavel Svoboda Audrey Combes ¹ Julia Petit Lucie Nováková Valérie Pichon ² Bmaals Group ³

1 ICAN - Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition

2 LSABM - Laboratoire Sciences Analytiques, Bioanalytiques, et Miniaturisation

3 NET - Neuroépidémiologie Tropicale

Abstract : The aim of the work was to synthesize a molecularly imprinted material for the selective solid-phase extraction (SPE) of β-N-methylamino-l-alanine (l-2-amino-3-methylpropionic acid; BMAA) from cyanobacterial extracts. BMAA and its structural analogs that can be used as template are small, polar and hydrophilic molecules. These molecules are poorly soluble in organic solvents that are commonly used for the synthesis of acrylic-based polymers. Therefore, a sol gel approach was chosen to carry out the synthesis and the resulting sorbents were evaluated with different extraction procedures in order to determine their ability to selectively retain BMAA. The presence of imprinted cavities in the sorbent was demonstrated by comparing elution profiles obtained by using molecularly imprinted silica (MIS) and non-imprinted silica (NIS) as a control. The molecularly imprinted solid-phase extraction (MISPE) procedure was first developed in a pure medium (acetonitrile) and further optimized for the treatment of cyanobacterial samples. It was characterized by high elution recoveries (89% and 77% respectively in pure and in real media).The repeatability of the extraction procedure in pure medium, in real medium and the reproducibility of MIS synthesis all expressed as RSD values of extraction recovery of BMAA were equal to 3%, 12% and 5%, respectively. A MIS capacity of 0.34µmol/g was measured. The matrix effects, which affected the quantification of BMAA when employing a mixed mode sorbent, were completely removed by adding a clean-up step of the mixed-mode sorbent extract on the MIS.

Type de document :

Article dans une revue

Domaine :

Sciences du Vivant [q-bio]

Sciences du Vivant [q-bio] / Santé publique et épidémiologie

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https://hal-unilim.archives-ouvertes.fr/hal-01245425
Contributeur : Elisabeth Grelier <>
Soumis le : jeudi 17 décembre 2015 - 10:59:44
Dernière modification le : jeudi 4 juillet 2019 - 11:00:08

Synthesis of a molecularly imprinted sorbent for selective solid-phase extraction of β-N-methylamino-l-alanine.

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Synthesis of a molecularly imprinted sorbent for selective solid-phase extraction of β-N-methylamino-l-alanine.

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