Performance Testing of PCR Assay in Blood Samples for the Diagnosis of Toxoplasmic Encephalitis in AIDS Patients from the French Departments of America and Genetic Diversity of Toxoplasma gondii: A Prospective and Multicentric Study.

Abstract : Toxoplasmic encephalitis in patients with AIDS is a life-threatening disease mostly due to reactivation of Toxoplasma gondii cysts in the brain. The main objective of this study was to evaluate the performance of real-time PCR assay in peripheral blood samples for the diagnosis of toxoplasmic encephalitis in AIDS patients in the French West Indies and Guiana. Adult patients with HIV and suspicion of toxoplasmic encephalitis with start of specific antitoxoplasmic therapy were included in this study during 40 months. The real-time PCR assay targeting the 529 bp repeat region of T. gondii was performed in two different centers for all blood samples. A Neighbor-Joining tree was reconstructed from microsatellite data to examine the relationships between strains from human cases of toxoplasmosis in South America and the Caribbean. A total of 44 cases were validated by a committee of experts, including 36 cases with toxoplasmic encephalitis. The specificity of the PCR assay in blood samples was 100% but the sensitivity was only 25% with moderate agreement between the two centers. Altered level of consciousness and being born in the French West Indies and Guiana were the only two variables that were associated with significantly decreased risk of false negative results with the PCR assay. Our results showed that PCR sensitivity in blood samples increased with severity of toxoplasmic encephalitis in AIDS patients. Geographic origin of patients was likely to influence PCR sensitivity but there was little evidence that it was caused by differences in T. gondii strains. ClinicalTrials.gov NCT00803621.
Type de document :
Article dans une revue
PLoS Neglected Tropical Diseases, Public Library of Science, 2016, 10 (6), pp.e0004790
Liste complète des métadonnées

https://hal-unilim.archives-ouvertes.fr/hal-01340447
Contributeur : Elisabeth Grelier <>
Soumis le : vendredi 1 juillet 2016 - 10:33:45
Dernière modification le : jeudi 2 août 2018 - 18:10:45

Identifiants

  • HAL Id : hal-01340447, version 1
  • PUBMED : 27355620

Collections

Citation

Daniel Ajzenberg, Isabelle Lamaury, Magalie Demar, Cyrille Vautrin, André Cabié, et al.. Performance Testing of PCR Assay in Blood Samples for the Diagnosis of Toxoplasmic Encephalitis in AIDS Patients from the French Departments of America and Genetic Diversity of Toxoplasma gondii: A Prospective and Multicentric Study.. PLoS Neglected Tropical Diseases, Public Library of Science, 2016, 10 (6), pp.e0004790. 〈hal-01340447〉

Partager

Métriques

Consultations de la notice

132