The Stringent Response Promotes Antibiotic Resistance Dissemination by Regulating Integron Integrase Expression in Biofilms

Abstract : Class 1 integrons are genetic systems that enable bacteria to capture and express gene cassettes. These integrons, when isolated in clinical contexts, most often carry antibiotic resistance gene cassettes. They play a major role in the dissemination of antibiotic resistance among Gram-negative bacteria. The key element of integrons is the integrase, which allows gene cassettes to be acquired and shuffled. Planktonic culture experiments have shown that integrase expression is regulated by the bacterial SOS response. In natural settings, however, bacteria generally live in biofilms, which are characterized by strong antibiotic resilience and by increased expression of stress-related genes. Here, we report that under biofilm conditions, the stringent response, which is induced upon starvation, (i) increases basal integrase and SOS regulon gene expression via induction of the SOS response and (ii) exerts biofilm-specific regulation of the integrase via the Lon protease. This indicates that biofilm environments favor integron-mediated acquisition of antibiotic resistance and other adaptive functions encoded by gene cassettes.
Type de document :
Article dans une revue
mBio, American Society for Microbiology, 2016, 7 (4), pp.e00868. 〈10.1128/mBio.00868-16〉
Liste complète des métadonnées

Littérature citée [72 références]  Voir  Masquer  Télécharger

https://hal-unilim.archives-ouvertes.fr/hal-01500749
Contributeur : Claire Douady <>
Soumis le : lundi 3 avril 2017 - 16:04:32
Dernière modification le : mercredi 28 février 2018 - 17:04:01
Document(s) archivé(s) le : mardi 4 juillet 2017 - 13:01:27

Fichier

mBio-2016-Strugeon-.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Emilie Strugeon, Valentin Tilloy, Marie-Cécile Ploy, Sandra Da Re. The Stringent Response Promotes Antibiotic Resistance Dissemination by Regulating Integron Integrase Expression in Biofilms. mBio, American Society for Microbiology, 2016, 7 (4), pp.e00868. 〈10.1128/mBio.00868-16〉. 〈hal-01500749〉

Partager

Métriques

Consultations de la notice

90

Téléchargements de fichiers

56