PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents—Synthesis, physicochemical characterization and in vitro evaluation

Abstract : We describe the synthesis of cellulose nanocrystals (CNCs) complexed to siRNA. PEI (600 Da) was first covalently attached to CNCs by reductive amination reaction, then CNCs-PEI were loaded with siRNA, resulting in the formation of CNCs-PEI-siRNA particles, strongly stabilized by ionic interactions. Efficient cellular uptake of these particles was monitored by ethidium bromide staining. Not only did CNCs-PEI show no cytotoxicity at the studied concentrations, but they also protected siRNA from degradation and favored its delivery into the cells. This siRNA (siRNA killer) is able to silence the expression of cell cycle genes and to induce cell death by apoptosis. Therefore, this study suggests that these CNCs-PEI are promising non-viral nanovehicles for siRNA delivery and for efficient anti-tumor strategy.
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https://hal-unilim.archives-ouvertes.fr/hal-01738117
Contributeur : Claire Douady <>
Soumis le : mardi 20 mars 2018 - 11:23:48
Dernière modification le : mercredi 21 mars 2018 - 01:23:26

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Gautier Mark Arthur Ndong Ntoutoume, Vincent Grassot, Frédérique Brégier, Julien Chabanais, Jean-Michel Petit, et al.. PEI-cellulose nanocrystal hybrids as efficient siRNA delivery agents—Synthesis, physicochemical characterization and in vitro evaluation. Carbohydrate Polymers, Elsevier, 2017, 164, pp.258 - 267. ⟨10.1016/j.carbpol.2017.02.004⟩. ⟨hal-01738117⟩

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