Oncogramme, a new promising method for individualized breast tumour response testing for cancer treatment.

Stéphanie Giraud 1 E. Loum 2, 3 Barbara Bessette 2, 3 V Fermeaux 3 Christophe Lautrette 1
1 ONCOMEDICS SAS
2 HCP - Homéostasie Cellulaire et Pathologies
3 Service d'Anatomie Pathologique [CHU Limoges]
Abstract : BACKGROUND: Breast cancer is the most widely spread cancer in the world, attracting much research and individualized tumour response testing (ITRT) methods are now used to individualize patient chemotherapeutic administrations. A new ITRT method was developed with optimized processing. MATERIALS AND METHODS: Breast tumour fragments were separated and the cells seeded in a foetal calf serum-free defined medium. After various chemotherapeutic treatments, cytotoxicity was determined by cell death detection with calcein acetoxymethyl and ethidium homodimer labelling. RESULTS: The culture medium allowed breast tumour cell proliferation in culture, while preventing fibroblastic cell survival. Moreover, the cell death analysis gave rise to a chemoresistance profile called an Oncogramme, with statistically significant values. CONCLUSION: The Oncogramme is a new ITRT method which can predict patient cell sensitivities to chemotherapeutics and should be validated by a new phase I clinical trial.
Keywords : breast cancer primary culture chemotherapeutics therapy personalization oncogramme individualized tumour response testing
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https://hal-unilim.archives-ouvertes.fr/hal-01820823
Contributeur : Philippe Sindou <>
Soumis le : vendredi 22 juin 2018 - 09:58:47
Dernière modification le : samedi 23 juin 2018 - 01:21:00

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  • HAL Id : hal-01820823, version 1
  • PUBMED : 21273591

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Stéphanie Giraud, E. Loum, Barbara Bessette, V Fermeaux, Christophe Lautrette. Oncogramme, a new promising method for individualized breast tumour response testing for cancer treatment.. Anticancer Research, International Institute of Anticancer Research, 2011, 31 (1), pp.139-145. ⟨hal-01820823⟩

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