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Article dans une revue
Oncogramme, a new promising method for individualized breast tumour response testing for cancer treatment.
Abstract : BACKGROUND:
Breast cancer is the most widely spread cancer in the world, attracting much research and individualized tumour response testing (ITRT) methods are now used to individualize patient chemotherapeutic administrations. A new ITRT method was developed with optimized processing.
MATERIALS AND METHODS:
Breast tumour fragments were separated and the cells seeded in a foetal calf serum-free defined medium. After various chemotherapeutic treatments, cytotoxicity was determined by cell death detection with calcein acetoxymethyl and ethidium homodimer labelling.
RESULTS:
The culture medium allowed breast tumour cell proliferation in culture, while preventing fibroblastic cell survival. Moreover, the cell death analysis gave rise to a chemoresistance profile called an Oncogramme, with statistically significant values.
CONCLUSION:
The Oncogramme is a new ITRT method which can predict patient cell sensitivities to chemotherapeutics and should be validated by a new phase I clinical trial.
https://hal-unilim.archives-ouvertes.fr/hal-01820823
Contributeur : Philippe Sindou Connectez-vous pour contacter le contributeur
Soumis le : vendredi 22 juin 2018 - 09:58:47
Dernière modification le : mercredi 20 octobre 2021 - 01:31:08
Contributeur : Philippe Sindou Connectez-vous pour contacter le contributeur
Soumis le : vendredi 22 juin 2018 - 09:58:47
Dernière modification le : mercredi 20 octobre 2021 - 01:31:08
