mRNA levels of enzymes and receptors implicated in arachidonic acid metabolism in gliomas

Rafaël de Armas 1, 2 Karine Durand 1 Angélique Guillaudeau 1 Nicolas Weinbreck 1 Sandrine Robert 1 Jean-Jacques Moreau 3 François Caire 3 Gisela Acosta 2 Matias Pebet 2 Alain Chaunavel 1 Benoît Marin 4, 5 François Labrousse 6 Yves Denizot 7
1 Service d'Hématologie biologique [CHU Limoges]
2 Hospital das Clinicas Montevideo
3 Service de Neurochirurgie [CHU Limoges]
4 SIME - Service de l'Information Médicale et de l'Évaluation [CHU Limoges]
5 NETEC - Neuroépidémiologie Tropicale et Comparée
6 HCP - Homéostasie Cellulaire et Pathologies
7 CRIBL - Contrôle de la Réponse Immune B et des Lymphoproliférations
Abstract : Background Gliomas are tumors of the central nervous system derived from glial cells. They show cellular heterogeneity and lack specific diagnostic markers. Although a possible role for the eicosanoid cascade has been suggested in glioma tumorigenesis, the relationship between enzymes and receptors implicated in arachidonic acid metabolism, with histological tumor type has not yet been determined. Design and methods Quantitative real-time reverse transcription-polymerase chain reaction was performed to measure and compare transcript levels of enzymes and receptors implicated in both lipoxygenase and cyclooxygenase pathways between oligodendrogliomas, astrocytomas, glioblastomas and mixed oligoastrocytomas. Results Arachidonic acid metabolism-related enzymes and receptor transcripts (i) were underexpressed in classical oligodendrogliomas compared to astrocytomas and/or glioblastomas, (ii) differed between astrocytomas and glioblastomas and (iii) had an intermediate expression in mixed oligoastrocytomas. Conclusions mRNA levels of enzymes and receptors implicated both in lipoxygenase and cyclooxygenase pathways differed significantly in gliomas according to the histological type.
Keywords : Cyclooxygenase Lipoxygenase Arachidonic acid metabolism Astrocytomas Oligodendrogliomas Gliomas Quantitative RT-PCR mRNA expression
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https://hal-unilim.archives-ouvertes.fr/hal-01820947
Contributeur : Philippe Sindou <>
Soumis le : vendredi 22 juin 2018 - 10:45:33
Dernière modification le : samedi 23 juin 2018 - 01:21:00

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Rafaël de Armas, Karine Durand, Angélique Guillaudeau, Nicolas Weinbreck, Sandrine Robert, et al.. mRNA levels of enzymes and receptors implicated in arachidonic acid metabolism in gliomas. Clinical Biochemistry, Elsevier, 2010, 43 (10-11), pp.827 - 835. ⟨10.1016/j.clinbiochem.2010.03.017⟩. ⟨hal-01820947⟩

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