Does Trypanosoma brucei brucei have the ability to invade human microglial cells?

Abstract : African trypanosomes are flagellated extracellular parasites, causal agents of African trypanosomiasis. Trypanosoma brucei is the most frequent species for the majority of African trypanosomiasis. Two sub-species are pathogenic for humans: Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense while Trypanosoma brucei brucei is not. Objective: The aim of this work was to study the infectivity of Trypanosoma brucei brucei, on human microglial cells (CMH-5). Methods: Trypanosomes (Trypanosoma brucei brucei Antat 1.9) labeled with a fluorescent dye were co-cultured with CMH-5 cells for 3 h. The nucleus and cytoskeletal microtubules of the cells were then labeled with two fluorescent dyes and the stained cell layer was examined by confocal microscopy. Results: Trypanosomes were observed within CMH-5 cells. Conclusion: Trypanosoma brucei brucei, a non-human parasite and described as an extracellular parasite, appears to have the ability to enter in human microglial cells in vitro. This raises a number of issues concerning the behaviour of this parasite in the human CNS and the immune response initiated by microglial cells which corresponds to endogenous defenses of the human brain.
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Contributeur : Elisabeth Grelier <>
Soumis le : jeudi 17 janvier 2019 - 11:42:55
Dernière modification le : jeudi 7 février 2019 - 15:29:28


  • HAL Id : hal-01984748, version 1



Julien Bonnet, Clotilde Boudot, Bertrand Courtioux. Does Trypanosoma brucei brucei have the ability to invade human microglial cells?. Archives of Parasitology, OMICS Group, 2017, 1 (1), pp.108. ⟨hal-01984748⟩



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