Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Development of Highly Reliable SERS‐active Photonic Crystal Fiber Probe and its Application in the Detection of Ovarian Cancer Biomarker in Cyst Fluid

Abstract : Conventionally Surface enhanced Raman spectroscopy (SERS) is realized by adsorbing analytes onto nano-roughened planar substrate coated with noble metals (silver or gold) or their colloidal nanoparticles (NPs). Nano scale irregularities in such substrates/NPs could lead to SERS sensors with poor reproducibility and repeatability. Herein, we demonstrate a suspended core photonic crystal fiber (PCF) based SERS sensor with extremely high reproducibility and repeatability in measurement with a relative standard deviation of only 1.5% and 4.6% respectively, which makes it more reliable than any existing SERS sensor platforms. In addition, our platform could improve the detection sensitivity owing to the increased interaction area between the guided light and the analyte, which is incorporated into the holes that runs along the length of the PCF. Numerical calculation established the significance of the interplay between light coupling efficiency and evanescent field distribution, which could eventually determine the sensitivity and reliability of the developed SERS active-PCF sensor. As a proof of concept, using this sensor, we demonstrated the detection of haptoglobin, a biomarker for ovarian cancer, contained within the ovarian cyst fluid, which facilitated in differentiating the stages of the cancer. We envision that with necessary refinements, this platform could potentially be translated as a next generation highly sensitive SERS-active opto-fluidic biopsy needle for the detection of biomarkers in body fluids.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal-unilim.archives-ouvertes.fr/hal-02414365
Contributeur : Sylvain Vedraine <>
Soumis le : lundi 14 décembre 2020 - 16:40:38
Dernière modification le : mardi 22 décembre 2020 - 13:44:38

Fichier

SERS Active PCF-Haptoglobin se...
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Flavien Beffara, Jayakumar Perumal, Aniza Puteri Mahyuddin, Mahesh Choolani, Saif Khan, et al.. Development of Highly Reliable SERS‐active Photonic Crystal Fiber Probe and its Application in the Detection of Ovarian Cancer Biomarker in Cyst Fluid. Journal of Biophotonics, Wiley, 2019, ⟨10.1002/jbio.201960120⟩. ⟨hal-02414365⟩

Partager

Métriques

Consultations de la notice

85

Téléchargements de fichiers

23