Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy

Daiki Kaneta (1) , Makiko Goto (2) , Motofumi Hagihara (2) , Philippe Leproux (3, 4) , Vincent Couderc (3) , Mariko Egawa (2) , Hideaki Kano (1, 5)

1 Université de Tsukuba = University of Tsukuba
2 S/PARK - Shiseido Global Innovation Center
3 XLIM - XLIM
4 LEUKOS
5 Kyushu University

We performed label-free imaging of human-hair medulla using multi-modal nonlinear optical microscopy. Intra-medulla lipids (IMLs) were clearly visualized by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging. Two groups of IMLs were found: second harmonic generation (SHG) active and inactive. By combining SHG analysis with CARS, the two groups were identified as free fatty acids and wax esters, respectively.

Domaines: Biotechnologies

Daiki Kaneta, Makiko Goto, Motofumi Hagihara, Philippe Leproux, Vincent Couderc, et al.. Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy. Analyst, 2021, 146 (4), pp.1163-1168. ⟨10.1039/D0AN01880E⟩. ⟨hal-03560542⟩