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Article Dans Une Revue Applied Physics Express Année : 2021

Visualization of water concentration distribution in human skin by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) microscopy

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Daiki Kaneta
  • Fonction : Auteur
Masahiro Kimura
  • Fonction : Auteur
Vincent Couderc
Makiko Goto
Mariko Egawa
Hideaki Kano

Résumé

Abstract Ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging was used to visualize the distribution of water concentration in human skin ex vivo. The CARS signal of the OH stretching vibrational mode of water was found to coexist with the signal of intercellular lipids such as ceramides, which were visualized by a sharp vibrational band at 2882 cm −1 . Depth-resolved CARS spectroscopic imaging of a skin sample revealed that ceramides were localized in the stratum corneum. These findings demonstrate the powerful potential of CARS spectroscopic imaging for probing pathological changes caused by anomalous water concentration distribution in human skin.
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Dates et versions

hal-03560584 , version 1 (07-02-2022)

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Daiki Kaneta, Masahiro Kimura, Philippe Leproux, Vincent Couderc, Makiko Goto, et al.. Visualization of water concentration distribution in human skin by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) microscopy. Applied Physics Express, 2021, 14 (4), pp.042010. ⟨10.35848/1882-0786/abee9f⟩. ⟨hal-03560584⟩
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