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Article Dans Une Revue Frontiers in Cellular and Infection Microbiology Année : 2021

Lichen Polyphenolic Compounds for the Eradication of Candida albicans Biofilms

Résumé

Lichens, due to their symbiotic nature (association between fungi and algae), constitute a chemical factory of original compounds. Polyphenolic compounds (depsides and depsidones) are the main constituents of lichens and are exclusively biosynthesized by these organisms. A panel of 11 polyphenols was evaluated for their anti-biofilm activity against Candida albicans biofilms on the maturation phase (anti-maturation) (MMIC 50 ) as well as on preformed 24-h-old biofilm (anti-biofilm) (MBIC 50 ) using the XTT assay. Minimum inhibitory concentrations of compounds (MICs) against C. albicans planktonic yeast were also determined using a broth microdilution method. While none of the tested compounds were active against planktonic cells (IC 50 > 100 µg/ml), three depsides slowed the biofilm maturation (MMIC 50 ≤12.5 µg/ml after 48 h of contact with Candida cells). Evernic acid was able to both slow the maturation and reduce the already formed biofilms with MBIC 50 ≤12.5 µg/ml after 48 h of contact with the biofilm. This compound shows a weak toxicity against HeLa cells (22%) at the minimal active concentration and no hemolytic activity at 100 µg/ml. Microscopic observations of evernic acid and optimization of its solubility were performed to further study this compound. This work confirmed the anti-biofilm potential of depsides, especially evernic acid, and allows to establish the structure–activity relationships to better explain the anti-biofilm potential of these compounds.
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hal-04032960 , version 1 (16-03-2023)

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Marion Girardot, Marion Millot, Guillaume Hamion, Jeanne-Louise Billard, Camille Juin, et al.. Lichen Polyphenolic Compounds for the Eradication of Candida albicans Biofilms. Frontiers in Cellular and Infection Microbiology, 2021, 11, pp.698883. ⟨10.3389/fcimb.2021.698883⟩. ⟨hal-04032960⟩
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