Protein Secondary Structure Imaging with Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy

Kotatsu Bito , Masanari Okuno , Hideaki Kano , Shilomi Tokuhara , Saturo Naito , Yoshinori Masukawa , Philippe Leproux (1) , Vincent Couderc (1) , H.-O. Hamaguchi

1 XLIM-PHOTONIQUE - PHOTONIQUE

Protein secondary structures in human hair have been studied with ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The CARS peak-shift mapping method has been developed and applied to hair samples with and without treatments by chemical reduction and mechanical extension. It clearly visualizes the treatment induced changes in protein secondary structures and their spatial distributions. Using the new imaging technique, we found a multilayered structure in the human hair cortex.

Kotatsu Bito, Masanari Okuno, Hideaki Kano, Shilomi Tokuhara, Saturo Naito, et al.. Protein Secondary Structure Imaging with Ultrabroadband Multiplex Coherent Anti-Stokes Raman Scattering (CARS) Microspectroscopy. Journal of Physical Chemistry B, 2012, 116 (4), pp. 1452-1457. ⟨hal-00746645⟩