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Article Dans Une Revue Toxicology Année : 2012

GSM-900 MHz at low dose temperature-dependently downregulates α-synuclein independently of chaperone-mediated-autophagy

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Résumé

The expanding use of GSM devices has resulted in public concern. Chaperone-mediated autophagy (CMA) is a way for protein degradation in the lysosomes and increases under stress conditions as a cell defense response. α-synuclein, a CMA substrate, is a component of Parkinson disease. Since GSM might constitute a stress signal, we raised the possibility that GSM could alter the CMA process. Here, we analyzed the effects of chronic exposure to a low GSM-900 MHz dose on apoptosis and CMA. Cultured cerebral cortical cells were sham-exposed or exposed to GSM-900 MHz at specific absorption rate (SAR): 0.25 W/kg for 24 h using a wire-patch cell. Apoptosis was analyzed by DAPI stain of the nuclei and western blot of cleaved caspase-3. The expression of proteins involved in CMA (HSC70, HSP40, HSP90 and LAMP-2A) and α-synuclein were analyzed by western blot. CMA was also quantified in situ by analyzing the cell localization of active lysosomes. 24 h exposure to GSM-900 MHz resulted in ∼0.5 °C temperature rise. It did not induce apoptosis but increased HSC70 by 26% and slightly decreased HSP90 (<10%). It also decreased α-synuclein by 24% independently of CMA, since the localization of active lysosomes was not altered. Comparable effects were observed in cells incubated at 37.5 °C, a condition that mimics the GSM-generated temperature rise. The GSM-induced changes in HSC70, HSP90 and α-synuclein are most likely linked to temperature rise. We did not observe any immediate effect on cell viability. However, the delayed and long term consequences (protective or deleterious) of these changes on cell fate should be examined.
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Dates et versions

hal-00760131 , version 1 (03-12-2012)

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Faraj Terro, Amandine Magnaudeix, Marion Crochetet, Ludovic Martin, Sylvie Bourthoumieu, et al.. GSM-900 MHz at low dose temperature-dependently downregulates α-synuclein independently of chaperone-mediated-autophagy. Toxicology, 2012, 292 (2-3), pp.PP. 136-144. ⟨10.1016/j.tox.2011.12.003⟩. ⟨hal-00760131⟩
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