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Article Dans Une Revue Sensors and Actuators A: Physical Année : 2015

Microfluidic Biosensors for Microwave Dielectric Spectroscopy

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Jonathan Leroy
  • Fonction : Auteur
  • PersonId : 919507
Fatima Hjeij
  • Fonction : Auteur
Alaeddine Landoulsi
  • Fonction : Auteur
  • PersonId : 949527
Carole Mélin
  • Fonction : Auteur
  • PersonId : 958520
Claire Dalmay
Barbara Bessette
  • Fonction : Auteur
  • PersonId : 905525
Christophe Bounaix Morand Du Puch
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  • PersonId : 949221
Stéphanie Giraud
Christophe Lautrette
  • Fonction : Auteur
  • PersonId : 949222
Serge Battu
  • Fonction : Auteur
  • PersonId : 901631
Fabrice Lalloué
Annie Bessaudou
Pierre Blondy
  • Fonction : Auteur
  • PersonId : 915831
Arnaud Pothier

Résumé

This paper deals with the development and characterization of label-free microfluidic biosensors ded-icated to the microwave dielectric spectroscopy of small volumes and flowing micro-scale particles.Especially, microwave dielectric spectroscopy offers unique capabilities to investigate intracellular prop-erties of biological cells, which are informative on many biological processes. This paper presents twoprototypes of high frequency biosensors able to operate in the microwave frequency range. Both arebased on capacitive detection either in a broadband or resonant mode. Thick gold metal electrodes havebeen investigated to perform high sensitive analysis, while a microfluidic system was implemented overthe RF circuit so that flowing liquid minimally impacts global losses of the system and has hence a limitedeffect on sensor sensitivity. Particles to be tested are manipulated to be accurately located in the detectionarea using dielectrophoresis techniques. To demonstrate the proposed approach and assess the detec-tion capabilities of such sensors, HF measurements (up to 8 GHz) have been performed on calibratedsize polystyrene beads (12 m diameter) and compared with full-wave electromagnetic simulations.Measured capacitance contrasts are typically around 1 fF at 5 GHz. Preliminary experimentations werethen achieved on cancerous cells. The presented results demonstrate the high potential of the proposedapproach to develop new label-free analysis tools with a large application range, either in the diagnosisor treatment fields.
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Dates et versions

hal-01244879 , version 1 (16-12-2015)

Identifiants

Citer

Jonathan Leroy, Fatima Hjeij, Alaeddine Landoulsi, Carole Mélin, Claire Dalmay, et al.. Microfluidic Biosensors for Microwave Dielectric Spectroscopy. Sensors and Actuators A: Physical , 2015, Volume 229, pp.Pages 172-181. ⟨10.1016/j.sna.2015.04.002⟩. ⟨hal-01244879⟩
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