UHF-Dielectrophoresis Crossover Frequency as a New Marker for Discrimination of Glioblastoma Undifferentiated Cells

Rémi Manczak (1) , Sofiane Saada (2) , Thomas Provent (1) , Claire Dalmay (1) , Barbara Bessette (2) , Gaëlle Bégaud (2) , Serge Battu (2) , Pierre Blondy (1) , Marie-Odile Jauberteau (2) , Canan Baristiran Kaynak , Mehmet Kaynak , Cristiano Palego (3) , Fabrice Lalloué (2) , Arnaud Pothier (1)

1 XLIM-RFEI - RF-ELITE : RF-Electronique Imprimée pour les Télécommunications et l'Energie
2 CAPTuR - Contrôle de l’Activation Cellulaire, Progression Tumorale et Résistance thérapeutique
3 Bangor University

This article introduces the first results of dielectric spectroscopy characterization of glioblastoma cells; measuring their crossover frequencies in the Ultra High Frequency range (above 50 MHz) by dielectrophoresis techniques. Experiments were performed on two glioblastoma lines: U87-MG and LN18 that were cultured following different conditions, in order to achieve different phenotypic profiles. We demonstrate here that the presented dielectrophoresis electrokinetic method can be used to discriminate the undifferentiated from the differentiated cells. In this study, microfluidic lab-on-chip systems implemented on Bipolar-Complementary Oxide Semiconductor (BiCMOS) technology are used allowing single cell handling and analysis. Based on characterizations of their own intracellular features, both selected glioblastoma cell lines cultured in distinct culture conditions have shown clear differences of DEP crossover frequency signatures compared to differentiated cells cultured in normal medium. These results support the concept and validate the efficiency for cell characterization in glioblastoma pathology.

Mots clés: BiCMOS chip, biological cell manipulation, glioblastoma cells, high frequency dielectrophoresis.

Domaines: Ingénierie biomédicale; Electromagnétisme; Cancer; Biophysique [physics.bio-ph]

Rémi Manczak, Sofiane Saada, Thomas Provent, Claire Dalmay, Barbara Bessette, et al.. UHF-Dielectrophoresis Crossover Frequency as a New Marker for Discrimination of Glioblastoma Undifferentiated Cells. IEEE Journal of Electromagnetics, RF and Microwaves in Medicine and Biology, In press, 3 (3), pp.191-198. ⟨10.1109/jerm.2019.2895539⟩. ⟨hal-02008203⟩